The method involves the near-infrared (NIR) laser irradiation of a thin film of carbon nanotubes, which act as an effective photon absorber as well as a stimulus generator.
Cell membranes can be manipulated by irradiating a thin film of carbon nanotubes with near-infrared (NIR) laser.
In cell engineering and tissue biology research, the use of pulsed lasers to stimulate cells has emerged as a powerful technique for enabling selective gene transfection, drug injection or the regulation of gene expression. The irradiation of biological cells using pulsed lasers causes their membranes to perforate, which significantly accelerates gene transfection or the targeted delivery of drugs.
Among the wide range of photon energies, the near-infrared region is less harmful for biological cells, which absorb very little energy in these wavelengths. The most successful NIR lasers are femtosecond lasers due to their fine spatial resolution with no thermal or mechanical damage to surrounding materials. But femtosecond laser instruments are expensive and require a highly sophisticated optical arrangement and much space, so the research team opted for a more economical nanosecond laser.
In the study, Naotoshi Nakashima and colleagues at Kyushu University used a dish coated with single-walled carbon nanotubes (SWCNTs), which strongly absorb radiation in the NIR region, as an antenna for a nanosecond pulse laser. The team found that cell membranes were either reversibly or irreversibly disturbed following an NIR pulse, depending on the energy of the laser. When a pulse exceeded 17.5 microJoules, the membrane was irreversibly disrupted and the cell died. By contrast, at about 15 microJoules per pulse, the membrane opened and the cell remained alive. This suggests an inexpensive laser source could be used to prepare a single cell target for selective gene transfection, drug injection or regulation of gene expression, the authors conclude.
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