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Posted: Dec 18, 2014
Creation of 'Rocker' protein opens way for new smart molecules in medicine
(Nanowerk News) Human cells are protected by a largely impenetrable molecular membrane, but researchers have built the first artificial transporter protein that carries individual atoms across membranes, opening the possibility of engineering a new class of smart molecules with applications in fields as wide ranging as nanotechnology and medicine.
The study, which appears Friday, Dec. 19, in the journal Science ("De novo design of a transmembrane Zn2+-transporting four-helix bundle"), is a milestone in designing and understanding membrane proteins. A PDF is available upon request. The study was conducted by researchers from Dartmouth College, the University of California-San Francisco, Massachusetts Institute of Technology and National Institute of Science Educational and Research in India.
Rocker (blue ribbons and yellow sticks) is an artificially designed protein that transports zinc ions (green) across biological membranes (gray sticks) by binding zinc ions it at one end of the molecule and rearranging ('rocking') to pass them onto the other end. The protein was built by researchers from Dartmouth College and other institutions. (Image: Dartmouth College)
Each human cell is surrounded by a lipid membrane, a molecular barrier that serves to contain the cellular machinery and protect it from the surrounding elements. This cellular "skin" is impenetrable to most biological molecules but also presents a conundrum: if chemicals can't get in or out, how is a cell to receive nutrients (food) and remove unwanted products of metabolism (trash)? Nature has come up with an elegant solution to this logistical problem -- transporter proteins (or transporters). These molecular machines are embedded in the cellular membrane and serve as gatekeepers, allowing specific chemicals to shuttle in and out when needed. Though biologists have known about transporters for many decades, their precise mechanism of action has been elusive.
The researchers set out to "build" an artificial transporter protein from scratch, to learn how transporters work, and to open the possibility of engineering a new class of smart molecules. They developed new computational techniques to model the necessary molecular physics, enabling them to design a transporter protein through computer simulation. Specifically, computer simulations suggested which amino-acid building blocks should comprise the future transporter, so that it would carry ionic atoms of metal zinc in one direction across membranes, while pumping protons in the other. Using this computational blueprint, they created the molecule in the lab, referring to it as "Rocker" due to its predicted molecular dynamic properties: the protein was expected to "rock" between two alternating states, allowing it to drive atoms through.
"To our great excitement, experiments showed that Rocker did indeed transport zinc and protons and it did, in fact, rock between two states just as designed," says co-lead author Gevorg Grigoryan, an assistant professor of computer science at Dartmouth. "Further, Rocker showed great selectivity, not transporting ions of calcium, another design feature."
Proteins are nature's workhorse molecules, performing a great variety of tasks in the cell from catalysis and sensing to generation of mechanical work. Learning to design (from first principles) novel protein molecules to perform specific tasks would mean that the immense richness of function that proteins have to offer can be brought to bear in a variety of applications, from better therapeutics to smart materials and clean energy solutions.
"Our findings are an important step forward in this pursuit, demonstrating that through the use of computer simulations to orchestrate precise properties of atomic structure and molecular dynamics, proteins can now be designed to carry out complex functions that rival those of natural molecular machines," Grigoryan says. "Further, our work represents a milestone in designing and understanding membrane proteins, a particularly challenging class of proteins."