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Posted: January 7, 2010

Unravelling Protein Puzzles. Malvern's Multiple Detection SEC Delivers Otherwise Unavailable Data

(Nanowerk News) A new presentation on the Malvern Instruments website discusses how the company's Viscotek TDAmax multiple detection size exclusion chromatography (SEC) system enables protein scientists to determine the degree of oligomerisation, aggregation, conformation, and conjugation in protein samples. By combining data from light scattering, UV, refractive index (RI), and viscometer detection, the TDAmax delivers efficient measurement of absolute molecular weight and molecular size, as well as information about protein structure that is simply not available using more traditional systems. The presentation can be viewed at http://www.malvern.com/proteintdsec.
Viscotek TDAmax
Viscotek TDAmax
The Viscotek TDAmax incorporates RI, light scattering and viscometer detectors, processing all three signals together at each data slice (5 Hz). By incorporating a combination of both low angle (7°) and right angle (90°) light scattering detectors, it can measure the absolute molecular weight (Mw) of a sample directly as it elutes from the column, without extrapolation or any additional modelling. Molecular weight, intrinsic viscosity and molecular size are determined across the entire distribution, state-of-the-art software enabling the results to be visualized graphically for ease of interpretation. The addition of a fourth detector, a UV or Diode Array UV, adds in compositional analysis – a very powerful tool for proteins scientists.
About Malvern Instruments
Malvern Instruments provides a range of complementary materials characterization tools that deliver inter-related measurements reflecting the complexities of particulates and disperse systems, nanomaterials and macromolecules. Analytical instruments from Malvern are used in the characterization of a wide variety of materials, from industrial bulk powders to nanomaterials and delicate macromolecules. A broad portfolio of innovative technologies is combined with intelligent, user-friendly software. These systems deliver industrially relevant data enabling our customers to make the connection between micro (such as particle size) and macro (bulk) material properties (rheology) and chemical composition (chemical imaging).
Particle size, particle shape, zeta potential, molecular weight, chemical composition and rheological properties measurements are now joined by advanced chromatography solutions (GPC/SEC), extending Malvern’s technologies for protein molecular weight, size and aggregation measurements, and synthetic polymer molecular weight and distribution. The company’s laboratory, at-line, on-line and in-line solutions are proven in sectors as diverse as cement production and pharmaceutical drug discovery.
Headquartered in Malvern, UK, Malvern Instruments has subsidiary organizations in all major European markets, North America, China, Korea and Japan, a joint venture in India, a global distributor network and applications laboratories around the world.
Source: Malvern Instruments (press release)
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