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Posted: April 17, 2010
RNA interference delivered using nanoparticles hits target in human patients
(Nanowerk News) A multi-institutional team of researchers and clinicians has published the first proof that a targeted nanoparticle can traffic into tumors, deliver double-stranded small interfering RNAs (siRNAs), and turn off the production of an important cancer protein using a mechanism known as RNA interference (RNAi). Moreover, the team provided the first demonstration that this new type of therapy, infused into the bloodstream, can make its way to human tumors in a dose-dependent fashion, that is, a higher number of nanoparticles sent into the body leads to a higher number of nanoparticles in the tumor cells. These two findings were achieved in phase I clinical trials in which the investigators are testing a nanoparticle-siRNA construct as an anticancer therapy.
These results, which were published in the journal Nature ("Evidence of RNAi in humans from systemically administered siRNA via targeted nanoparticles"), demonstrate the feasibility of using both nanoparticles and RNAi-based therapeutics in patients, and open the door for future "game-changing" therapeutics that attack cancer and other diseases at the genetic level, says team leader Mark E. Davis of the California Institute of Technology. Dr. Davis is also a member of the Nanosystems Biology Cancer Center, a National Cancer Institute Center for Cancer Nanotechnology Excellence.
The discovery of RNAi, the mechanism by which double strands of RNA silence genes, won researchers Andrew Fire and Craig Mello the 2006 Nobel Prize in Physiology or Medicine. The scientists first reported finding this novel mechanism in worms in a 1998 Nature paper. Since then, the potential for this type of gene inhibition to lead to new therapies for diseases such as cancer has been highly touted.
"RNAi is a new way to stop the production of proteins," says Dr. Davis. What makes it such a potentially powerful tool, he adds, is the fact that its target is not a protein, the typical target for anticancer drugs. The vulnerable areas of a protein may be hidden within its three-dimensional folds, making it difficult for many therapeutics to reach them. In contrast, RNA interference targets the messenger RNA (mRNA) that encodes the information needed to make a protein in the first place.
"In principle," says Dr. Davis, "that means every protein now is druggable because its inhibition is accomplished by destroying the mRNA. And we can go after mRNAs in a very designed way given all the genomic data that are and will become available."
Still, there have been numerous potential roadblocks to the application of RNAi technology as therapy in humans. One of the most problematic has been finding a way to ferry the therapeutics, which are made up of fragile siRNAs, into tumor cells after direct injection into the bloodstream. Dr. Davis, however, had a solution. Even before the discovery of RNAi, he and his team had begun working on ways to deliver nucleic acids to cells via the blood stream. They eventually created a four-component system, featuring a unique polymer called cyclodextrin, that self-assembles in the presence of RNA into a targeted, siRNA-containing nanoparticle. The siRNA delivery system is under clinical development by Calando Pharmaceuticals, Inc., based in Pasadena, California.
"These nanoparticles are able to take the siRNAs to the targeted site within the body," says Dr. Davis. Once they reach their target, in this case, the cancer cells within tumors, the nanoparticles enter the cells and release the siRNAs.
As part of their study, the team was able to detect and image nanoparticles inside cells biopsied from the tumors of several of the phase I trial's participants. In addition, Dr. Davis and his colleagues were able to show that the higher the nanoparticle dose administered to the patient, the higher the number of particles found inside the tumor cells—the first example of this kind of dose-dependent response using targeted nanoparticles. Even better, Dr. Davis says, the evidence showed the siRNAs had done their job. In the tumor cells analyzed by the researchers, the mRNA encoding the cell-growth protein ribonucleotide reductase – the target of the siRNA encapsulated in the nanoparticle – had been degraded. This degradation, in turn, led to a loss of the protein.
More to the point, the mRNA fragments found were exactly the length and sequence they should be if they'd been cleaved in the spot targeted by the siRNA, notes Dr. Davis. "It's the first time anyone has found an RNA fragment from a patient's cells showing the mRNA was cut at exactly the right base via the RNAi mechanism," he says. "It proves that the RNAi mechanism can happen using siRNA in a human."
Source: National Cancer Institute
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