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Posted: Sep 30, 2014
Next-generation sequencer applications: Innovation to turn 'junk DNAs' into genetic markers
(Nanowerk News) A series of the Next-Generation Sequencer (NGS) applications are being developed to utilize ‘junk DNAs’ as distinctive genetic analysis systems for crop species.
High-throughput sequencing of retrotransposon insertion sites and its applications.
Many nucleic DNAs do not have any known biological functions. Such DNAs are referred to as ‘junk DNAs’. Having accumulated genomic copies throughout evolution, retrotransposons constitute a major portion of junk DNAs. Although most copies have lost copy-making ability, small groups of the retrotransposons still remain competent. The copies of an active group are inserted at markedly different genomic positions, even among closely related varieties of the crop species.
Identification of the active groups is, however, challenging as they are concealed by numerous non-active groups. Additionally, DNA sequences of the numerous positions where active groups are inserted must be efficiently determined for genetic population under study.
The series of NGS applications on active retrotransposons provide efficient genetic analysis systems, especially for variety fingerprinting, linkage map construction, and lineage analysis of the crop species.