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Posted: Sep 25th, 2012
Researchers develop new technique for identifying proteins secreted by cells
(Nanowerk News) Researchers from North Carolina State University have developed a new technique to identify the proteins secreted by a cell. The new approach should help researchers collect precise data on cell biology, which is critical in fields ranging from zoology to cancer research.
The work is important because cells communicate by secreting proteins. Some of the proteins act on the cell itself, telling it to grow or multiply, for example. But the proteins can also interact with other cells, influencing them to perform any biological function.
Traditionally, scientists who wanted to identify these proteins cultured cells and then used mass spectrometry to determine which proteins appeared in the medium the cell was grown on. This has drawbacks, because the proteins of interest are fairly rare compared to the proteins that are already in the medium – which are used to grow and support the cells in the first place. Further, any attempts to culture the cells without these background, supporting proteins affects cell behavior – skewing the sample.
The new approach takes advantage of the fact that each cell “packages” its proteins in its “secretory pathway.” Each cell synthesizes the protein and passes it through this pathway, essentially placing it in a bag-like membrane before it is passed out of the cell.
“This gives us a snapshot of exactly what a cell was secreting at that point in time,” says Dr. Balaji Rao, an assistant professor of chemical and biomolecular engineering at NC State and co-author of a paper describing the work.
This new method eliminates the problems related to the proteins found in cell culture media. But it also allows researchers to track changes in the proteins released by a cell in response to a stimulus, such as exposure to a chemical. This can be done by taking samples at various points in time after cells have been exposed to the stimulus.
And, in principle, this technique would also allow researchers to identify which proteins any specific type of cell is secreting when in a mixed population of cells.
“As long as you can separate the cells you are interested in, this should be possible,” says Rao. “And that is important, because most tissues are made up of heterogeneous populations of cells – and communication between those cells is biologically significant.”
Source: North Carolina State University
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