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Posted: May 18, 2012
Dolomite Develops Novel Droplet System for Encapsulation of Single Cells or Beads
(Nanowerk News) Dolomite, a world leader in the design and
manufacture of microfluidic devices, has successfully developed an innovative
Droplet System for the encapsulation of single cells or beads into droplets
which benefits a wide range of applications including single cell analysis, high
throughput screening and droplet PCR.
The system represents a major breakthrough in microfluidics by providing users
with the tools required to perform assays on individual cells in picolitre volume
environments. This novel technology was demonstrated by feeding 2.8µm diameter
magnetic beads into monodisperse 100µm diameter droplets at a ratio of 1 bead per
droplet with minimal bead losses.
2.8µm diameter beads inside monodisperse droplets (100µm in diameter).
Featuring Dolomite's standard microfluidic components, the new system allows users
to load single cells or beads into droplets. The Mitos P-Pump allows stirring of the
input fluid reservoir, to provide an even distribution of beads and delivers a pulseless and stable flow for excellent control of droplet volumes. Reliable and leak-free
connections are made from pump to tube to chip using Dolomite's Multiflux® Linear
Droplets are isolated in an inert fluorocarbon carrier stream to prevent crosscontamination
and are stabilized with Pico-Surf™, a biocompatible surfactant
designed specifically for generating and stabilizing pico-droplets.
For further information on Dolomite's Micro Droplet Systems as well as the complete
portfolio of microfluidic products including chips, connectors/ interconnects, valves,
pumps and custom devices please visit www.dolomite-microfluidics.com.
The Dolomite Centre Limited
Established in 2005 as the world's first Microfluidic Application Centre, Dolomite focused on working with
customers to turn their concepts for microfluidic applications into reality. Today, Dolomite is the world
leader in solving microfluidic problems. With offices in the UK and US and distributors throughout the rest
of the world, its clients range from universities developing leading-edge analytical equipment, to
manufacturers of chemical, life science and clinical diagnostics systems.
Dolomite is pioneering the use of microfluidic devices for small-scale fluid control and analysis, enabling
manufacturers to develop more compact, cost-effective and powerful instruments. By combining specialist
glass, quartz and ceramic technologies with knowledge of high performance microfluidics, Dolomite is able
to provide solutions for a broad range of application areas including environmental monitoring, clinical
diagnostics, food and beverage, nuclear, agriculture, petrochemical, cosmetics, pharmaceuticals and
chemicals. Furthermore Dolomite's in-house micro-fabrication facilities that include clean rooms and
precision glass processing facilities allow to prototype and test all solutions rapidly which ensures a faster
development cycle and reduces the time to market. For more information please visit www.dolomite-microfluidics.com.