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Posted: September 9, 2008
NanoSight Announces Significant Software Advances for Their Instruments
(Nanowerk News) NanoSight Limited, the nanoparticle characterization company, has announced significant software advances for their family of instrumentation. New software versions usually offer fixes to problems and slight tweaks to existing routines in response to user requests. These advances provide particular benefits to users to improve the validity of their measurements.
Extended Analysis answers two key questions for users of NTA (Nanoparticle Tracking Analysis): How long should a user run an analysis and how many particles does the user need to measure to get a statistically good result? The two are interdependent: the longer the analysis time, the more particles are counted, as the contents of the interrogated volume self-refresh through diffusion. NTA 2.0 now analyses particles as they are detected, eliminating the need to record for post experiment analysis thus speeding use and repeatability. Users may now run analyses far longer and improve the development of the optimal analysis time.
Extended Dynamic Range is achieved through the interleaving of alternate video frames at high and then low gain settings will help users track large and small particles in the same sample, thus greatly increasing dynamic range. For example, four clear peaks are readily observed for a mix of latex reference particles of 50, 200, 400 and 590nm.
The 3D diameter scatter plot clearly showing resolution of 100nm and 200nm particles with the inference of the presence of dimers of the 100nm nanoparticles
The 3D Diameter Scatter Plot shows diameter plotted against scatter intensity to both boost resolution and provide a capability to differentiate similar sized particles when they are of differing materials. In these analyses, a third axis plots scatter intensity against the existing particle size distribution of number count vs. size. In the figure shown, the analysis allows the presence of dimer particles to be seen and contrasted to slightly larger but significantly brighter particles.
The last new benefit is derived from Model-Fitting. This enhancement compensates for the natural broadening of displayed results. This broadening is inherent in the stochastic nature of Brownian motion and the physical limitation of only being able to track particles for a finite number of frames. Model-Fitting works by compensating for the number of tracks (consecutive jumps, frame to frame) used in the NTA analysis. This effectively compensates for the Gaussian breadth of the curve where its origin is understood. Users are able to apply this feature to specific peaks displayed in the particle size analysis. The background to this development was described by Professor Paul Van der Meeren in his presentation at the PSA 2008 conference held in Stratford-upon-Avon.
While current users will benefit from these developments through receiving a free upgrade, these and other routines have been introduced with all new systems at no increased charge reflecting NanoSight’s commitment to be at the leading edge in instrumentation for nanoparticle characterization.
For more information about NanoSight and how their instrumentation is applied to applications in both the biological and materials sciences, please visit www.nanosight.co.uk.
NanoSight Ltd, of Salisbury, UK, is the world leading provider of instruments for the optical detection and real time analysis of sub-micron particles. The Company supplies unique instruments for nanoparticle analysis in the sub-micron region that go far beyond existing light scattering techniques in characterising polydispersed systems. The company currently has over 100 systems in service worldwide having begun commercial sales in 2006.
The Company’s proprietary knowledge and expertise has enabled the delivery of technologies to blue chip companies and universities for direct visualisation of individual nanoscale particles in suspension from which independent quantitative estimation of particle size, size distribution and concentration are immediately obtained. The Company has recently launched additions to its advanced Nanoparticle Tracking Analysis (NTA) software that build on the ground-breaking work of NanoSight’s LM20 and LM10 systems.