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Human non B cells are depleted by incubating the sample with the Human Pan B cell isolation optimized biotin conjugated antibody cocktail mixture followed by incubation with magnetic streptavidin nanoNanoparticles. The magnetically labeled antibody is pulled out by the use of a magnetic separator to leave the target cells intact in the supernatant. The untouched total human B cells are collected by decanting the liquid in a clean tube. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. These magnetic nanoparticles can be used wiht both free standing magnets and column-based systems. https://www.cd-bioparticles.com/p/Pan-B-Cell-Isolation-Kit-Human_6_198_198_5432.html