Enhancing biomarker detection with manganese oxide nanozymes

(Nanowerk News) A recent publication in Sensors and Actuators: B. Chemical ("Laser-generated defect-rich MnOx nanobelts with high oxidase mimic activity for glutathione detection") showcases the creation of highly potent oxidase-mimicking nanozymes through the use of MnOx nanobelts (NBs) produced by laser irradiation in liquid (LIL) techniques. The research was conducted by scientists from the Institute of Solid State Physics, Hefei Institute of Physical Sciences, and the Chinese Academy of Sciences.
While oxidase-mimic nanozymes have demonstrated potential in biomarker detection, their limited activity compared to natural enzymes has hindered broader adoption.
The researchers discovered that ultrathin layered MnOx NBs enhance the exposure of catalytic active sites, while the negatively charged layer of birnessite-type MnOx NBs strengthens affinity for positively charged substrates such as 3,3',5,5'-tetramethylbenzidine (TMB).
Growth mechanism of the ultrathin manganese oxide nanobelts
Growth mechanism of the ultrathin MnOx NBs. (Image: JI Shihan)
Notably, the creation of oxygen vacancies through laser irradiation lowered the MnOx NBs' oxygen adsorption energy, yielding exceptional substrate affinity (Km = 0.0087 mM) and a high catalytic rate (Vmax = 6.04 × 10-7 M/s).
Moreover, the team leveraged glutathione (GSH) inhibition of nanozymes with oxidase (OXD) mimics to devise a rapid and highly sensitive GSH detection method.
These insights may pave the way for novel strategies in synthesizing highly active nanozymes for biomarker applications.
Source: Hefei Institutes of Physical Science (Note: Content may be edited for style and length)
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