|Posted: Nov 29, 2012|
From rotting mushroom to drug?
|(Nanowerk News) Soft rot diseases cause a great deal of damage in agriculture, and turn fruits, vegetables, and mushrooms to mush. By using imaging mass spectrometry together with genetic and bioinformatic techniques (genome mining), German researchers have now discovered the substance the bacteria use to decompose mushrooms. As the scientists report in the journal Angewandte Chemi ("Imaging Mass Spectrometry and Genome Mining Reveal Highly Antifungal Virulence Factor of Mushroom Soft Rot Pathogen")e, the substance called jagaricin could represent a starting point for the development of new antifungal drugs.|
|Caught in the act: Imaging mass spectrometry of a button mushroom (see picture) infected with the soft rot pathogen Janthinobacterium agaricidamnosum in conjunction with genome mining revealed jagaricin as a highly antifungal virulence factor that is not produced under standard cultivation conditions. The structure of jagaricin was rigorously elucidated by a combination of physicochemical analyses, chemical derivatization, and bioinformatics.|
|Button mushrooms with soft rot develop typical lesions and are eventually completely disintegrate. The pathogen causing soft rot in cultivated mushrooms has been identified as Janthinobacterium agarididamnosum. A team led by Christian Hertweck at the Leibniz Institute for Natural Product Research and Infection Biology in Jena (Germany) wanted to know which bacterial compound is responsible for this destruction in order to better understand the pathobiology and to find possible protective measures. If the soft rot bacteria produce a substance that attacks mushrooms, it is also conceivable that this substance could be effective against microbial fungi, which cause dangerous infections in humans.|
|Their challenge was to search for an unknown substance that the bacteria do not produce under standard culture conditions, but only when they attack a mushroom. Hertweck and his co-workers used a method called genome mining. They sequenced the genome of the bacterium and searched it for relevant biosynthesis genes. Using bioinformatic techniques, they made predictions about the structures of the metabolites. In order to stimulate the production of virulence factors, the researchers infected slices of button mushroom with the bacterium and examined the resulting decomposed areas with imaging mass spectrometry. This produced a mass spectrum for every point measured on the analyzed surface, allowing the researchers to identify a mass peak that only occurred in spectra from infected areas. The team found a DNA jag sequence that codes for the biosynthetic apparatus that produces the substance in question. Addition of mushroom fragments and a special growth medium then allowed them to obtain and isolate larger amounts of the compound from a liquid cell culture.|
|The structure of jagaricin – which is what they called the substance – was fully determined using physical chemical analyses, chemical derivatization, and bioinformatics. The compound is a novel lipopeptide with an unusual structure. Pure jagaricin induces the symptoms of soft rot in mushrooms. The researchers were thus able to demonstrate that jagaricin is involved in the infectious process of the soft rot disease. Degrading enzymes presumably also participate.|
|The scientists also determined that jagaricin is effective against Candida albicans, Aspergillus fumigatus, and Aspergillus terreus, which cause human fungal infections. Perhaps this substance could be a starting point for the development of a new antifungal drug.|
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