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Posted: November 10, 2008
Researchers stimulate patchiness in phospholipid membranes with charged nanoparticles
(Nanowerk News) Like water and ice cubes mixed in a glass, a group of organic compounds called lipids can coexist as liquid and solid in membranes. This patchiness in phospholipid membranes is fundamental to their use as biomolecules and biosensors.
Using charged nanoparticles, researchers at the University of Illinois have found a new way to stimulate patchiness in phospholipid membranes.
"We are seeing a previously unsuspected responsiveness in phospholipid membranes," said Steve Granick, a Founder Professor of Engineering at the U. of I. "What we thought was possible only with the specificity of certain proteins, we now see can happen with simple, charged nanoparticles."
Lipids are the building blocks of cell membranes. In earlier work, Granick and graduate student Liangfang Zhang found a way to stabilize sensitive lipid membranes by attaching charged nanoparticles to the membrane surface.
Steve Granick, a Founder Professor of Engineering at the U. of I., and colleagues have found that a group of organic compounds called lipids can coexist as liquid and solid in membranes. This patchiness in phospholipid membranes is fundamental to their use as biomolecules and biosensors.
Now, Granick, Zhang, graduate research assistant Bo Wang and research scientist Sung Chul Bae show that a phospholipid membrane can coexist in two phases – solid and liquid – according to what binds to it. This inherent patchiness presents an additional mechanism for changing the stiffness of phospholipid membranes.
The researchers report their work in a paper to be published next week in the Online Early Edition of the Proceedings of the National Academy of Sciences.
Using fluorescence and calorimetry methods, the researchers studied interactions between charged nanoparticles and membranes formed from single-component lipids. Because the membrane was composed of one sole lipid type, the traditional explanation for spatial patchiness – an uneven distribution of different lipids – was eliminated.
While a variety of nanoparticles was used, the most common type was polystyrene spheres about 20 nanometers in diameter (a nanometer is 1 billionth of a meter). Where the nanoparticles attached to the membrane, the membrane responded by changing phase.
"The electric charge acted as a switch," Granick said. "Nanoparticles with a negative charge switched membranes from liquid to solid. Nanoparticles with a positive charge switched the membranes from solid to liquid."
Phase changes occurred in patches of membranes where phospholipid molecules swiveled after binding to charged nanoparticles. This binding-induced behavior, where the same lipid can coexist in two different phases, offers a new mechanism for modulating stiffness in membranes.
In future work, the researchers plan to study the effects of smaller, charged nanoparticles; the effects of charged nanoparticles on living cells; and novel ways to stabilize lipid membranes for targeted drug delivery.
"These experiments are helping us better understand both the structure of phospholipid membranes and the potential biological effects of exposure to nanoparticles found in our normal, everyday environment," Granick said.
Source: University of Illinois at Urbana-Champaign