The authors point out that they synthesized the ZnO nanorods on the activated glass slides in a low-cost and scalable batch process that does not depend on cleanroom facilities.
To improve the interaction on the ZnO nanorod surface, herringbone structures were incorporated inside the microfluidic device for enhanced mixing.
The team demonstrated highly selective detection of H5N2 subtype AIV by fast continuous flow in the microfluidic channels that effectively minimizes nonspecific adsorption.
The entire detection process could be completed within 1.5 hours, while the limit of detection (LOD) for the H5N2 AIV was measured as 3.6 × 103 EID50 mL-1.
This platform shows the capability for simultaneous detection of multiple viral pathogens by spatial encoding different antibodies onto the same ZnO-NIM.
Moreover, the scientists further demonstrated the captured H5N2 AIV could be released by simply dissolving the ZnO nanorods in acidic environment and the released H5N2 AIV could be detected by the real-time RT-PCR (rRT-PCR).
The successful virus release enables subsequent off-chip analyses of the captured viruses by ZnO-NIM, which complements to the on-chip multiplexed sandwich immunoassay and greatly expands its capability.
As the authors note, this sensitive, selective, multiplex portable technology and the integrated fluorescence sandwich immunoassay can be expanded to detect other pathogens and disease biomarkers.