Crystals of membrane proteins and protein complexes often diffract to low resolution owing to their intrinsic molecular flexibility, heterogeneity or the mosaic spread of micro-domains. At low resolution, the building and refinement of atomic models is a more challenging task. The deformable elastic network refinement method developed previously has been instrumental in the determination of several structures at low resolution. Here, DEN refinement is reviewed.
Researchers have developed a new cocktail that coaxes adult cells to become pluripotent stem cells of a high enough quality to be used in therapeutic applications. Their research showed that using a different combination of reprogramming factors can produce a much higher quality result, delivering fewer colonies of iPSCs of which 80 percent passed the toughest pluripotency test.
Scientists have identified a highly sensitive means of analyzing very tiny amounts of DNA. The discovery, they say, could increase the ability of forensic scientists to match genetic material in some criminal investigations. It could also prevent the need for a painful, invasive test given to transplant patients at risk of rejecting their donor organs and replace it with a blood test that reveals traces of donor DNA.
Researchers have developed a new approach to measure proteins with structures that change. This could enable new diagnostic tools for the early recognition of neurodegenerative diseases to be developed.
Researchers have resolved a long-standing challenge in stem cell biology by successfully 'resetting' human pluripotent stem cells to a fully pristine state, at point of their greatest developmental potential.
The recently developed fluorescent protein Amrose is now being used for advanced near-IR imaging procedures. With the aid of a novel evolutionary platform technology, scientists have developed this infrared marker as part of a combined effort to improve the quality of tissue imaging.
Another mystery of the human body has been solved by scientists who have identified how a molecular motor essential for human development works. They have also pinpointed why mutations in genes linked to this motor can lead to a range of human diseases.