Self-assembly is Nature's way of building stuff. This fundamental principle that governs natural structures on all scales, from molecules to galaxies, generates structural organization from pre-existing parts or components. In nanotechnology, self-assembly is seen as a key technique that will one day allow the fabrication of materials and devices from the bottom up. Still only tinkering with the basics, scientists so far have designed and created simple systems that could mimic natural functions by connecting biological components to abiotic materials to understand the workings of the biological system or to take advantage of the unique properties of the nonbiological components in a natural setting. Most nanotechnologist, even if they manage to self-assemble functional nanodevices, still operate exclusively at the nanoscale (it will be a while before you can order "Tea. Earl Grey. Hot" from your food replicator in the wall). Bridging the gap between the nano- and the macroworld has proven to be a huge hurdle. In a novel approach that merges material chemistry, biology and medicine, researchers in Germany have used living bacteria to show that self-assembly of functional materials and living systems is possible through a chemically programmed construction.
Freshwater looks like it will become the oil of the 21st century - scarce, expensive and fought over. While over 70 per cent of the Earth's surface is covered by water, most of it is unusable for human consumption. According to the Government of Canada's Environment Department (take a look at their Freshwater Website - a great resource for facts and all kinds of aspects about water), freshwater lakes, rivers and underground aquifers represent only 2.5 per cent of the world's total freshwater supply. Unfortunately, in addition to being scarce, freshwater is also very unevenly distributed. The United Nations has compared water consumption with its availability and has predicted that by the middle of this century between 2 billion and 7 billion people will be faced with water scarcity. It gets worse: In the developing countries, 80 per cent of illnesses are water-related. Due to the shortage of safe drinking water in much of the world, there are 3.3 million deaths every year from diarrheal diseases caused by E. coli, salmonella and cholera bacterial infections, and from parasites and viral pathogens. In fact, between 1990 and 2000, more children died of diarrhea than all the people killed in armed conflicts since the Second World War. The use of nanotechnologies in four key water industry segments - monitoring, desalinization, purification and wastewater treatment - could play a large role in averting the coming water crisis. But hoping that the 'magic' of nanotechnology will solve all water problems is naive - the basic problems of accessibility to technologies, affordability, and fair distribution still need to be solved.
Vaccination has resulted in the eradication of smallpox and control of measles, rubella, tetanus, diphtheria, and other infectious diseases in many areas of the world (at least where vaccines are available and affordable; providing vaccines to many parts of the developing world still is one of the basic medical needs that is far from being met). The basic idea of vaccination (the word comes from the Latin vacca - cow - because the first vaccine was derived from a virus affecting cows) is to inject weakened or killed forms of pathogens such as bacteria or viruses into the body in order for the immune system to develop antibodies against them; if the same types of microorganisms enter the body again, they will be destroyed by the antibodies. About 25 years ago, the basic idea of vaccination gave rise to bactofection - the technique of using bacteria as non-viral gene carriers into target cells. The DNA cargo is transported inside the bacteria and, once it arrives at the target location, the bacteria is broken up in order to release the therapeutic gene or protein. A novel technique takes advantage of the invasive properties of bacteria for delivery of nanoparticles into cells. Here, the gene or cargo is not carried inside the bacteria, but rather remains on the surface conjugated to nanoparticles. Consequently, this approach does not require bacterial disruption for delivery, or any genetic engineering of the bacteria for different cargo.
In the early 1870s, the German physicist Ernst Karl Abbé formulated a rigorous criterion for being able to resolve two objects in a light microscope. According to his equation, the best resolution achievable with visible light is about 200 nanometers. This theoretical resolution limit of conventional optical imaging methodology was the primary factor motivating the development of recent higher-resolution scanning probe techniques. The interaction of light with an object results in the generation of what is called 'near-field' and 'far-field' light components. The far-field light propagates through space in an unconfined manner and is the visible light utilized in conventional light microscopy. The near-field (or evanescent) light consists of a nonpropagating field that exists near the surface of an object at distances less than a single wavelength of light. So called near-field microscopy beats light's diffraction limit by moving the source very close to the subject to be imaged. When the first theoretical work on a new technique called "scanning near-field optical microscopy" (SNOM or NSOM) appeared in the 1980's, Abbé's classical diffraction limit was overcome, and resolution even down to single molecule level became feasible. However, light microscopy is still the only way to observe the interior of whole, or even living, cells. The use of fluorescent dyes makes it possible to selectively obtain images of individual cell components, for example, proteins. Today, the wavelength dogma has been overcome with the development of the stimulated emission depletion (STED) microscope. Now, the German team that developed STED is reporting layer-by-layer light microscopic nanoscale images of cells and without having to prepare thin sections with a technique called optical 3D far-field microscopy. They use a chemical marker for fluorescence nanoscopy that relies on single-molecule photoswitching.
A revolutionary new environmental biotechnology - the Microbial Fuel Cell - turns the treatment of organic wastes into a source of electricity. Fuel cell technology, despite its recent popularity as a possible solution for a fossil-fuel free future, is actually quite old. The principle of the fuel cell was discovered by German scientist Christian Friedrich Schoenbein in 1838 and published in 1839. Based on this work, the first fuel cell was developed by Welsh scientist Sir William Robert Grove in 1843. The operating principle of a fuel cell is fairly straightforward. It is an electrochemical energy conversion device that converts the chemical energy from fuel (on the anode side) and oxidant (on the cathode side) directly into electricity. Today, there are many competing types of fuel cells, depending on what kind of fuel and oxidant they use. Many combinations of fuel and oxidant are possible. For instance, hydrogen cell uses hydrogen as fuel and oxygen as oxidant. Other fuels include hydrocarbons and alcohols. An interesting - but not commercially viable yet - variant of the fuel cell is the microbial fuel cell (MFC) where bacteria oxidize compounds such as glucose, acetate or wastewater. Researchers in Spain have fabricated multi-walled carbon nanotube (MWCNT) scaffolds with a micro-channel structure in which bacteria can grow. This scaffold structure could be used as electrodes in microbial fuel cells.
Carbon comes in many different forms, from the graphite found in pencils to the world's most expensive diamonds. In 1980, we knew of only three basic forms of carbon, namely diamond, graphite, and amorphous carbon. Then, fullerenes and carbon nanotubes were discovered and all of a sudden that was where nanotechnology researchers wanted to be. Recently, though, there has been quite a buzz about graphene. Discovered only in 2004, graphene is a flat one-atom thick sheet of carbon. Existing forms of carbon basically consist of sheets of graphene, either bonded on top of each other to form a solid material like the graphite in your pencil, or rolled up into carbon nanotubes (think of a single-walled carbon nanotube as a graphene cylinder) or folded into fullerenes. Physicists had long considered a free-standing form of planar graphene impossible; the conventional wisdom was that such a sheet always would roll up. Initially using such high-tech gadgets like pencils and sticky tape to strip chunks of graphite down to layers just one atom thick, the process has now been refined to involve more expensive instruments such as electron beam and atomic force microscopes. Despite being isolated only three years ago, graphene has already appeared in hundreds of papers. The reason scientists are so excited is that two-dimensional crystals (it's called 2D because it extends in only two dimensions - length and width; as the material is only one atom thick, the third dimension, height, is considered to be zero) open up a whole new class of materials with novel electronic, optical and mechanical properties.
"Children begin to learn by seeing, hearing, tasting and, above all, by touching. In a very similar approach, we are currently learning to orient ourselves in the nanoworld by 'feeling' materials - not with our fingers, but with microscopes that allow us to probe these materials with atomic resolution." (Robert W. Stark, LMU Munich in "Getting a feeling for the nanoworld"). Researchers' ability to engineer materials and achieve superior electronic, thermal, magnetic, and mechanical properties depends on tools that can identify and characterize material components and their spatial arrangement at the nanoscale. Equally important, understanding structure and function relationships in biological systems also demands tools that can probe structural properties with molecular resolution. Atomic force microscopes (AFM) are the most widely used tools to image matter at the nanoscale. Due to its mechanical operation, the AFM can in principle also perform nanomechanical measurements. This aspect of the AFM has been explored by researchers over the past two decades. However, current state-of-the-art techniques are very slow (it takes about one second for the AFM tip to approach, push into and retract from the surface of a material) and they apply rather large forces during the measurement process that damage the tip and the sample. Researchers at Harvard and Stanford universities have developed a specially designed AFM cantilever tip, the torsional harmonic cantilever (THC), which offers orders of magnitude improvements in temporal resolution, spatial resolution, indentation and mechanical loading compared to conventional tools. With high operating speed, increased force sensitivity and excellent lateral resolution, this tool facilitates practical mapping of nanomechanical properties.
One of the many fascinating concepts in nanotechnology is the vision of molecular electronics. If realized, the shift in size from even the smallest computer chip today would be staggering - a quantum leap, so to speak (literally). Look at it this way: a single drop of water contains more molecules than the billions and billions of silicon chips ever produced. Molecular electronics engineers of tomorrow might use individual molecules to perform the functions in an electronic circuit that are performed by semiconductor devices today. Don't get your hopes up, though, that your next iPod will be truly nano. Scientists today are still struggling with the most basic requirements for molecular electronics, for instance, how to precisely and reliably position individual molecules on a surface. DNA-based nanostructuring is one approach that could lead to promising results. It has already been shown that DNA could be used to structure nanoscale surfaces. Now, a team in Germany has demonstrated that nanoscale objects of very different size can be deposited simultaneously and site-selectively onto DNA-displaying surfaces, based on sequence-specific DNA-DNA duplex formation.